Do you take TRT, and you’d like to check the impact on your health? Symptoms of an imbalance in these hormones include water retention, gynaecomastia (man-boobs) low libido, weight gain, erectile dysfunction, muscle loss, and infertility. Although primarily thought of as female hormones, oestradiol, progesterone, and prolactin are also found in males in lesser amounts. Elevated levels of these hormones in men can be a result of taking testosterone replacement (TRT). Symptoms of an imbalance in these hormones include water retention, gynaecomastia (man-boobs), low libido, weight gain, erectile dysfunction, muscle loss, and infertility. Oestradiol (a form of oestrogen) is a steroid hormone needed by women and men. While testosterone is thought of as the most significant male hormone, it needs to be in balance with oestradiol to help control sex drive, erectile function, and sperm production. Progesterone is produced by the corpus luteum and adrenal glands. In men, it is the precursor to testosterone and supports hormonal balance. Prolactin is produced by a small organ at the base of the brain called the anterior pituitary gland. Prolactin is best known for producing breast milk in females and for sexual function in men. Please take your sample before 10am. Take this test two to five days after the start of your period, ideally on day three. It can be taken any time if you do not have periods. Hormonal contraception can affect this test, taking a break from this and using barrier contraception will give more accurate results. If you use hormone gels, pessaries, patches, or tablets, we strongly recommend selecting a venous sample to minimise contamination.